Prerequisites:

Addressed to researchers already familiar with R and basics of statistics

The following packages are required:

  • RNAseq123
  • edgeR
  • Mus.musculus
  • limma
  • Glimma
  • RColorBrewer

Description:

In the BAD DAY 3 we will present the Bioconductor work-flow R package RNAseq123 from http://www.bioconductor.org/help/workflows/RNAseq123/.

We will in particular discuss how to:

  • Import and organise data
  • Preprocess data
  • Perform exploratory plots
  • Analyse Differential Expression in genes
  • Perform Exploratory analysis and graphical representation of DE genes
  • Test for Gene set enrichment

Outline/ content